Vegfa Knockout cell line (Raw 264.7)

Gene Editing Services

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Vegfa Knockout cell line (Raw 264.7) Order

Catalog#

YKO-MT0036

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Vegfa Knockout cell line (Raw 264.7)

Species

Mouse

Gene Name

Vegfa

Gene ID

22339

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 1 (based on transcript Vegfa-207)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Vegfa gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Vegfa gene knockout Vegfa Knockout cell line (Raw 264.7) will be expanded and cryopreserved.